CEBI Center for Biotechnology
Interdisciplinary Centre for Biotechnology employs researchers from differentfield of biotechnology, microbiology, physics and chemical engineering active on wide biotechnology area. Laboratory has fully equipped biosafety level 2 laboratory and facility for bioprocessing operations. Laboratory is conducting basic and applied research and it’s offering bioprocess contract development services.
Basic and applicative research on bacteriophages
Antibiotic-resistant bacteria are a serious global problem and there is an urgent need for new antibiotics or alternative approaches to prevent and treat bacterial infections. One of the most promising alternatives are bacteriophages—viruses able to invade different bacterial species as their specific host. The use of bacteriophages to treat and prevent bacterial infections has several advantages over antibiotics such as specificity, low environmental impact, auto-dosing, low inherent toxicity, lack of cross-resistance with antibiotics, formulation and application versatility, and safety for humans, plants, and animals. At the same time, there are some concerns such as narrow host range and the need for precise bacteria identification before bacteriophage application, resistance development, human immune response to bacteriophages, and so on. Research in Laboratory for Bio-Analytics is directed towards key issues of bacteriophage application, efficacy, and safety. As part of the project we are isolating and characterizing bacteriophages or their enzymes against important human, animal and plant pathogen bacteria of the genus Campylobacter, Salmonella, Escherichia coli, Staphylococcus, and Dickeya. The final goal of the project are bacteriophage-based products and tools that will enable effective contamination control in the food chain and treatment of diseases in humans, animals and plants.
Development and optimization of biomolecules production processes
Biological macromolecules—mainly proteins, nucleic acids, and also viruses—are widely used in various branches of industry, research, and medicine. Their production and analytics are complex and require constant improvements to ensure safe, economic, and regulatory-compliant production. CEBI has substantial expertise in developing upstream and downstream processes for biomolecules and viruses. On this basis, the laboratory team is offering competent process development for proteins, plasmid DNA, live and inactivated viral vaccines, and virus-like particles. The processes developed are GMP-compliant and scalable from the laboratory level, through the pilot level, and up to production level.
Biosensors for virus detection and characterisation
Virus quantification in particular could be a bottleneck in process development but also in regular production because traditional virology methods do not match the data accuracy needed for efficient process optimization and method validation. Having in mind this burden we are developing biosensors for viruses and virus-like particle analysis. Biosensors will be applicable in production processes of viral vaccines, viral vectors, and bacteriophages, where fast, accurate, and reliable in-process methods are needed.
ARRS L4-4277 Innovative production systems for vaccines and regenerative medicine
Centre for Biotechnology (CEBI) is cooperating in development of convective based supports for efficient chromatographic purification of cells, virus like particles and bacteriophages. Research is directed towards optimization of existing methacrylate monoliths and development of fractal chromatographic support. For purification of eukaryotic and prokaryotic cells a new generation of support will be developed, based on inorganic porous support, hollow fibres and macroporous chromatographic monoliths. In the frame of the project LBA is testing developed convective supports with cells, virus like particles and various bacteriophages.
Bilateral project Slovenia-Brasil 2012-2014:
»Development and use of aptamer analytical tools for tumor diagnostics based on circulation of tumor (stem) cells in blood«
The project's aim is to research and develop analytical and preparative tools for tumor cell diagnostics. BILAB's role in the project is to develop aptamers selective for circulating tumor (stem) cells which will be useful for the development of preparative and diagnostic tools.
The project is a cooperation between:
- Sao Paulo University, Brasil
- National institute of biology (NIB)
European regional development fund's Project Mina
»Environmental pollutants and neurodegenerative illnesses - a multidisciplinary study based on research and innovativity« in the framework of the European cross-border cooperation Italy-Slovenia 2007-2013.
The goal of the project is to support research and create synergies between research institutions in the field of biomedical studies and neurodegenerative illnesses. The project is focused on creating a new network of highly skilled staff that will have an improved access to joined infrastructure. Furthermore the researchers shall enjoy an enhanced mobility environment and the availability of new research themes that have considerable impact on social and health aspects of neurodegenerative illnesses. The support goes along the lines of research and development of biomedical technologies which could be used for the economic development of the region and could carry stimulus for more competitive businesses.
CEBI's role in the project:
- Research in the field of aptamer based biosensors for detection of adenosine triphosphate (ATP) and other soluble mediators that are important in neurological studies.
- Italy: International School for Advanced Studies (SISA), University of Udine
- Slovenija: Cobik, University of Nova Gorica
»Cross-border study of proteins for oncologic research and diagnosics« is a European regional development fund's project in the framework of the European cross-border cooperation Italy-Slovenia 2007-2013.
The project proposes setting up a center for automated production of recombinant proteins by means of cooperation between research institutions and small and medium businesses in Italy and Slovenia. The Center will be focused on a set of research project on general and applicative oncology. In the long run, the center will offer innovative, high-tech services. It will be open for the needs of academic and industrial researchers from biomedical, pharmaceutical and environmental scientific branches and will increase productivity and competitiveness. The synergy based on private-public partnership will enable to establish a training program on biotechnological and biomedical fields. The training will give rise to interdisciplinary and inter-sectorial knowledge.
CEBI's role in the project:
- Development of aptamers, aptamer markers and bio-receptors for bio-analytical applications for cancer research.
- Italy: Sincrotrone Trieste S.C.p.A., Interuniversity Consortium for Biotechnology (CIB) University of Udine.
Tovarniška 26, 5270 Ajdovščina
phone: 00386 05 8500 890
Head: Matjaž Peterka
Deputy: Aleš Podgornik